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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10ML
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文献和实验Purification of Plasmid from 50 ml-culture
well.) 17. Spin at 14,000 rpm for 5 min. Discard the supernatant. Rinse the pellet with 300 ul of cold 70% ethanol. Discar the fluid. Dissolve the pellet in 400 ul of TE. (Optional: Repeat PEG precipitation once more. This is recommended for preparing
Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
1. Prepare a Lysis Mixture by mixing one volume of Buffer MRB and one volume of isopropanol. For 96 samples, combine15 ml Buffer MRB with 15 ml isopropanol and mix throughly by vortexing. Add 300 ul Lysis Mixture for each sample at step 3. 2. Add 50 ul
PEG PRECIPITATION OF PCR PRODUCTS
1. After PCR, add the following reagents to each tube: For 30 µl reaction add: For 50 µl reaction add: 2. . After adding the PEG, stir pipet in tube and pipet up and down to mix. Make sure all solution remaining in the tip is injected
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