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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken 5' exonuclease Apollo,DCLRE1B
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken 5' exonuclease Apollo, DCLRE1B ELISA KIT
ELI-46705c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken 5' exonuclease Apollo, DCLRE1B ELISA KIT is for the quantitative in vitro determination of Chicken DCLRE1B concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken 5' exonuclease Apollo, DCLRE1B ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken DCLRE1B in the sample, this Chicken 5' exonuclease Apollo, DCLRE1B ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken DCLRE1B concentration. The concentration of Chicken DCLRE1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验一般PCR方法在扩增大片段DNA时的局限性: 通常所用的PCR方法都在两个方面 有局限,即目标产物精确程度 和合成片段的大小 。Pfu(Pyrococcus furiosus)DNA聚合酶, 具有完整的3'外切酶校读活性(3'-editing-exonuclease) ,可以将每个循环中碱基的错配率由10*-4降到10*-3,从而提高PCR产物的准确性。但它在扩增1.5-2.0kb片段时,效率比Klentaq l (Taq DNA聚酶N-末端缺失突变体,类似
’)2/Fab 部分反应。它们经过了 ELISA 和 / 或 Fc 片段的吸附检测。它们不是特异性 IgG ,因为它们是和轻链反应的,它们同时也和具有相同轻链的其他种类的免疫球蛋白( Ig D, IgM, IgA 和IgE )和亚类反应。 (min X …Sr Prot) 与一个物种反应的二抗可能与其他物种发生交叉反应,除非这类抗体经过其他物种的吸收实验。这类抗体经过了检测,以及针对 IgG 和圆括号中的物种的血清蛋白的吸附处理。 (标有“ (min X…Prot)” 字样的抗体是经过了
mol/L 硼酸和 1 mmol/L EDTA。 ( 7 ) 溴化乙锭(EB) 溶液:5 mg/ml EB 水溶液。 ( 8 ) 1% 的琼脂糖凝胶:1% ( m/V ) 的琼脂糖溶解在 0.5 X TBE 缓冲液中。加热至琼脂糖完全溶解。倒胶前加入 TB ( 1 : 10000 稀释)。在 0.5X TBE 缓冲液中电泳。 ( 9 ) 胶回收试剂盒(GE 或 Qiagen 公司)。 ( 10 ) PCR 回收试剂盒(GE 或 Qiagen 公司)。 2.3 酶解反应
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