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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Acetoacetyl-CoA synthetase,AACS
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Acetoacetyl- CoA synthetase, AACS ELISA KIT
ELI-24717c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Acetoacetyl- CoA synthetase, AACS ELISA KIT is for the quantitative in vitro determination of Chicken AACS concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Acetoacetyl- CoA synthetase, AACS ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken AACS in the sample, this Chicken Acetoacetyl- CoA synthetase, AACS ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken AACS concentration. The concentration of Chicken AACS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验酶分类的主要类别之一。为催化与 ATP等三磷酸核苷的分解伴有形成新化学键可逆反应的酶之总称。催化具有有机化学意义的合成反应,分为两个分子相加的裂解酶和移换反应的移换酶两类。所以还是将不伴随腺苷三磷酸的分解而催化合成反应的酶称为合成酶( Synthase)较为适宜。合成酶催化的反应含 ATP等基质,反应复杂, ATP分解为 ADP与正磷酸或 AMP与焦磷酸。根据生成键的种类而更分为:( 1) C-O键,例如氨酰 tRNA合成酶;( 2) C-S键,例如乙酰 CoA合成
脂肪酸合成酶 fatty acid synthetase 乙酰 CoA + 7 丙 = 酸 CoA + 14NADPH + 14H →棕榈酸 7CO 2 + 8CoA 14NADP 6H 2 O 。 催化上述反应的酶称为脂肪酸合成酶,不过酵母酶的最终产物是棕榈酸 CoA 。如图所示,在丙二酸基和乙酰基缩合时,在每次延长 C 2 单位的同时发生还原反应,在这个复杂的反应中,各有相应的酶参与作用。而酰基以 CoA 转移到酰基载体
A synthetase. Drug Metab. Dispos. 24:879‐883. Kelley, M. and Vessey, D.A. 1990. The effects of ions on the conjugation of xenobiotics by the aralkyl‐CoA and arylacetyl‐CoA N
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