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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken DNA replication licensing factor MCM3,MCM3
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken DNA replication licensing factor MCM3, MCM3 ELISA KIT
ELI-16343c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken DNA replication licensing factor MCM3, MCM3 ELISA KIT is for the quantitative in vitro determination of Chicken MCM3 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken DNA replication licensing factor MCM3, MCM3 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken MCM3 in the sample, this Chicken DNA replication licensing factor MCM3, MCM3 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken MCM3 concentration. The concentration of Chicken MCM3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
, 1; barrier to autointegration factor (BCRP1), mRNA 1303 NM_000214 Homo sapiens jagged 1 (Alagille syndrome) (JAG1), mRNA 536 NM_002167 Homo sapiens inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3), mRNA 1192 NM_001664 Homo
AcNPV ,Autographa califorinica nuclear polyhedrosis virus,苜蓿银纹夜蛾核型多角体病毒 att ,attachmet site,接受位点 Apr ,Ampicillin replication sequence,氨卞青霉素抗性基因 ARS ,Autonomors replication sequence,自主复制序列 Adv ,Adenovirus,腺病毒 atRNA
下游基因的表达,而下游基因的变化又可以通过基因芯片(cDNA Microarray),抑制消减杂交(Suppression Subtractive Hybridization),差异显示RT-PCR等方法进行研究[1]。然而这些方法都无法提供证据证明这些变化是受某个蛋白因子直接调节的,还是间接的其他变化导致的结果。所以,要想提供蛋白因子直接调控的证据,就要直接检测蛋白质-DNA的相互作用。传统的方法包括转录因子结合实验(Transcription Factor Assay),电泳迁移率变动分析
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