- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-15697c
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Signal peptide peptidase-like 2B,SPPL2B
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Signal peptide peptidase- like 2B, SPPL2B ELISA KIT
ELI-15697c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Signal peptide peptidase- like 2B, SPPL2B ELISA KIT is for the quantitative in vitro determination of Chicken SPPL2B concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Signal peptide peptidase- like 2B, SPPL2B ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken SPPL2B in the sample, this Chicken Signal peptide peptidase- like 2B, SPPL2B ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken SPPL2B concentration. The concentration of Chicken SPPL2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing
across the cytoplasmic membrane, the signal peptide is proteolytically removed by signal peptide cleavage. Here, in this chapter, we describe methods to study signal peptide processing in bacteria, including purification of signal peptidase and its substrates
Peptide Inhibition ELISA Protocol
Concentrated Sulfuric Acid88.0 ml di-H2O B. Procedure Titer MAb supes at serial 5-fold dilutions to determine the concentration at which the titer begins to drop. Dilute the MAb to one-half the above dilution in PBT
Rules for antigenic peptide selection by wxscmu1.Use more than one peptide2.Use 15-20 AA peptide3.Avoid signal peptide, transmembrane region. Avoid conserved region among both orthologs and paralogs (Do BLAST).4.Use the N- and C-terminal sequences
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