- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-12676c
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Peptidase inhibitor 15,PI15
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Peptidase inhibitor 15, PI15 ELISA KIT
ELI-12676c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Peptidase inhibitor 15, PI15 ELISA KIT is for the quantitative in vitro determination of Chicken PI15 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Peptidase inhibitor 15, PI15 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken PI15 in the sample, this Chicken Peptidase inhibitor 15, PI15 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken PI15 concentration. The concentration of Chicken PI15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验-1 Inhibitor Screening Assay Kit 目录号：218734 包装：96wells 本试剂盒为您提供了一种测量Caspase-1 及其他具有YVAD 酶切识别序列的蛋白酶活性的途径。Caspase-1 可以底物肽C 端的p-NA 切割下来而显色（405nm）。试剂盒以非常方便的96 孔酶标板的形式提供，也适用于对Caspase-1 的抑制剂的筛选。试剂盒中提供：重组人的Caspase-1 标准品 (Cat. No. 218783), 发色caspase-1 底物(Ac
. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。 11. 测定：以空白空调零，450nm 波长依序测量各孔的吸光度（OD 值）。 测定应在加终止 液后15 分钟以内进行。 操作程序总结： 计算 人一氧化氮(NO)酶联试剂盒说明书 以标准物的浓度为横坐标，OD 值为纵坐标，在坐标纸上绘出标准曲线，根据样品的 OD 值由标准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD 值计算出标 准曲线的直线回归方程式，将样品的OD 值
Proteasome Preparation from Human Brain
7) Assay fractions for protein and chymotrypsin (or other peptidase) activity:- a) Add 5ul sample (or 10ul if protein concentrations are low) to a 4ml tube. b) Add 100ul of working substrate solution. c) Incubate for 15 minutes at 37°C. d) Stop
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