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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Cytosolic 5'-nucleotidase III,NT5C3
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Cytosolic 5'- nucleotidase III, NT5C3 ELISA KIT
ELI-12251c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Cytosolic 5'- nucleotidase III, NT5C3 ELISA KIT is for the quantitative in vitro determination of Chicken NT5C3 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Cytosolic 5'- nucleotidase III, NT5C3 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken NT5C3 in the sample, this Chicken Cytosolic 5'- nucleotidase III, NT5C3 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken NT5C3 concentration. The concentration of Chicken NT5C3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验家族 (KRAB-ZFPs)。这两个 标记均在失活的 X 染色体上发现,其中,H3K27me3 位于基因间和沉默的编码区,H3K9me3 主要位 于活性基因的编码区。 酶调节 组蛋白甲基化是一种可以通过多次细胞分裂而仍然保持的稳定标记,多年来一直被认为不可逆。但是, 最新研究发现,它是一个主动调控和可逆的过程。 甲基化:组蛋白甲基转移酶 (HMTs) 赖氨酸 包含 SET 结构域(组蛋白尾部) 包含非 SET 结构域(组蛋白核心) 精氨酸 PRMT(蛋白
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
节省了抗体测试的时间和精力,优化的 protocol 大大降低了 ChIP 实验的难度、减少摸索实验条件的时间。经过不断的改良及完善,新一代的 EZ-ChIP 能够提供阴性、阳性对照和 DNA 纯化柱,使 ChIP 试验更为高效可靠,大量经典文献的引用即是其品质的保证 [27-29] 。另外,对于应用越来越多的 ChIP on CHIP,默克密理博还提供 EZ-Magna ChIP 试剂盒,磁珠分离技术让 ChIP 跨入高通量时代,为全基因组的探索插上翱翔的翅膀。 参考文献: [1] Zhang
of interest. In addition, include a control precipitation with an (IgG) antiserum that is not directed against chromatin proteins. Cell culture from which nuclei are to be extracted (1 x 107 to 1 x 108 cells are required; see Steps 9-16) Cell culture medium
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