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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Gallinacin-4,GAL4
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Gallinacin- 4, GAL4 ELISA KIT
ELI-08119c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Gallinacin- 4, GAL4 ELISA KIT is for the quantitative in vitro determination of Chicken GAL4 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Gallinacin- 4, GAL4 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken GAL4 in the sample, this Chicken Gallinacin- 4, GAL4 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken GAL4 concentration. The concentration of Chicken GAL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验说明书为准。 保存条件及有效期 纤维素 fitc流式ZNF286IHC、FACS、WB、E 1.试剂盒保存:;2-8℃。 2.有效期:6 个月 人一氧化氮(NO)酶联试剂盒说明书 2-甲氧乙基酯 fitc流式ZNF537IHC、FACS、WB、ELISA Rabbit polyclonal to ZNF537 antibody 乙二胺 fitc流式ZNF5IHC、FACS、WB、ELISA Rabbit
酵母单杂交技术 1.酵母单杂交的基本原理 酵母 单杂交技术是1993年由酵母双杂交技术发展而来的,其基本原理为:真核生物基因的转录起始需转录因子参与,转录因子通常由一个DNA特异性结合功能域和一个或多个其他调控蛋白相互作用的激活功能域组成,即DNA结合结构域(DNA―bindingdomain,BD)和转录激活结构域(activationdomain,AD)。用于酵母单杂交系统的酵母GAL4蛋白是一种典型的转录因子,GAL4的DNA结合结构
蛋白相互作用的激活功能域组成,即DNA结合结构域(DNA—bindingdomain,BD)和转录激活结构域(activationdomain,AD)。用于酵母单杂交系统的酵母GAL4蛋白是一种典型的转录因子,GAL4的DNA结合结构域靠近羧基端,含有几个锌指结构,可激活酵母半乳糖苷酶的上游激活位点(UAS),而转录激活结构域可与RNA聚合酶或转录因子TFIID相互作用,提高RNA聚合酶的活性。在这一过程中,DNA结合结构域和转录激活结构域可完全独立地发挥作用。据此,我们可将GAL4的DNA结合结构域置换为文库
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