- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-02235c
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken BH3-interacting domain death agonist,BID
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken BH3- interacting domain death agonist, BID ELISA KIT
ELI-02235c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken BH3- interacting domain death agonist, BID ELISA KIT is for the quantitative in vitro determination of Chicken BID concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken BH3- interacting domain death agonist, BID ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken BID in the sample, this Chicken BH3- interacting domain death agonist, BID ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken BID concentration. The concentration of Chicken BID in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验相关专题 细胞凋亡专题 线粒体 凋亡途径是细胞凋亡的主要途径之一，是目前研究凋亡的热点.各种凋亡刺激信号通过BH3(Bcl-2 homology domain 3)-only蛋白引起Bax(Bcl-2-asslciated protein X)蛋白移位到线粒体外膜并多聚化,形成膜通道,刺激线粒体释放细胞色素C(Cyt C) 和Smac(second mitochondrial-derived activator
. In many cases, information from the environment is relayed to a central 'apoptosis rheostat' at the mitochondrion by stimulus-specific induction, modification or movement of pro-apoptotic 'BH3 domain-only' members of the Bcl-2 family (including
和BH3结构域亚族。 (一)Bcl-2亚族 Bcl-2是这一亚族的代表。Bcl-2蛋白由羧基端疏水性的跨膜结构域(transmembrane domain，TM)和4个Bcl-2同源结构域(Bcl-2 homology，BH)组成。TM结构域使线粒体定位于细胞内膜上，其中线粒体外膜是Bcl-2存在的主要场所。失去TM结构域的Bcl-2不具有抑制凋亡的活性，提示Bcl-2的定位对其功能执行至关重要。BH结构域是Bcl-2与其他Bcl-2家族成员或细胞凋亡调控蛋白相互作用的基础，其中BHl
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