- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- CELI-66084c
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Folic acid,FA
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Folic acid, FA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Folic acid,Folacin,Vitamin B9,Vitamin Bc, folate, vitamin M, vitamin B9,vitamin Bc,folacin, pteroyl-L-glutamic acid,
pteroyl-L-glutamate,pteroylmonoglutamic acid, FA
Search name
Chicken Folic acid ELISA Kit, Chicken Folacin ELISA Kit, Chicken Vitamin B9 ELISA Kit, Chicken folate ELISA Kit, Chicken
vitamin M ELISA Kit, Chicken vitamin B9 ELISA Kit, Chicken vitamin Bc ELISA Kit, Chicken pteroyl-L-glutamic acid ELISA Kit,
Chicken pteroylmonoglutamic acid ELISA Kit, Chicken FA ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Chicken folic acid,Folic acid concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Folic acid, During the reaction, Folic acid in the sample or standard competes with a fixed amount
of biotin-labeled Folic acid for sites on a pre-coated Monoclonal antibody specific to Folic acid. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Folic acid in the samples is then determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验一种水溶性 B族维生素，是蝶呤的衍生物。最初是从菠菜叶中发现的，其结构中有取代的喋呤、对氨基苯甲酸和谷氨酸 3个部分，在肝、酵母和绿色植物中最丰富。烹饪时或接触还原剂时被破坏。在细胞内的活性形式是四氢叶酸（ tetrahydrofolicacid， THFA），它作为辅酶参与一碳基团的转移反应，在这些反应中，甲基（ -CH3 ）、甲酰基（ -CHO）、亚甲基（ -CH2 -）、甲川基（ -CH=）或亚甲氨基（ -CH=NH）等含一个碳原子的基团从一个分子转移到另一个分子
叶酸 folic acid 维生素 B复合体之一，相当于蝶酰谷氨酸（ pteroylglutamic acid， PGA）。是米切尔（ H． K． Mitchell， 1941）从菠菜叶中提取纯化的，而命名为叶酸。有促进骨髓中幼细胞成熟的作用，如果缺少叶酸可招致红血球的异常未成熟细胞的增加和贫血以及白血球减少。这是因为叶酸参与核酸的嘧啶和嘌呤的合成。此外也作为干酪乳杆菌（ Lactobacillus casei）及其它微生物促进增殖因子而起作用。与维生素 M是同一物质。已知 PGA
FA Extraction of [insoluble] Amyloid beta from Mouse Brain
实验试剂 FA Neutralization Solution (1M Tris base, 0.5 M Na2 HPO4 , 0.05% NaN3 ) 1. 60.57 g Tris base 2. 35.50 g Na2 HPO4 3. 2.5 ml 10% NaN3 4. H2 O to 500 ml; pH is not adjusted; store and use
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