- ￥3480
- LSM BIO
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-33428d
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测狗血清，血浆等样本中目标蛋白的浓度
- 适应物种：
狗
- 标记物：
Canine Bromodomain-containing protein 2,BRD2
- 样本：
狗的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Canine Bromodomain-containing protein 2, BRD2 ELISA KIT
Product Name:Canine Bromodomain-containing protein 2, BRD2 ELISA KIT
Packing:96T
Catalog No.:ELI-33428d
Gene Name:Canine Bromodomain-containing protein 2, BRD2
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine Bromodomain-containing protein 2, BRD2
Alternative Name:Bromodomain-containing protein 2,Bromodomain-containing protein 2, BRD2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Bromodomain-containing protein 2, BRD2 ELISA KIT allows for the in vitro quantitative determination of Canine Bromodomain-containing protein 2, BRD2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Bromodomain- containing protein 2, BRD2 ELISA KIT 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms BRD2,D6S113E; FSH; FSRG1; NAT; RING3; RNF3; O27.1.1; bromodomain-containing 2; female sterile homeotic-related gene 1; really interesting new gene 3 protein; bromodomain containing 2 Search name Canine BRD2 ELISA KIT ,Canine D6S113E ELISA KIT ,Canine FSH ELISA KIT ,Canine FSRG1 ELISA KIT ,Canine NAT ELISA KIT ,Canine RING3 ELISA KIT ,Canine RNF3 ELISA KIT ,Canine O27.1.1 ELISA KIT ,Canine bromodomain-containing 2 ELISA KIT ,Canine female sterile homeotic-related gene 1 ELISA KIT ,Canine really interesting new gene 3 protein ELISA KIT ,Canine bromodomain containing 2 ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of Canine Bromodomain- containing protein 2, BRD2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canine Bromodomain- containing protein 2, BRD2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Canine Bromodomain- containing protein 2, BRD2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Bromodomain- containing protein 2, BRD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Canine Bromodomain- containing protein 2, BRD2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Customized product Delivery time 4-6month
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验Protein Carbonyl Measurement by ELISA
) (2,3). Protein-bound DNP can also be measured with increased sensitivity by enzyme-linked immunosorbent assay (ELISA) (4,5). Samples containing protein are reacted with DNP, then the protein is nonspecifically adsorbed to an ELISA plate. Unconjugated DNP
Protein A Purification of Antibody
fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L
fusion protein present in your samples. This is the protocol for general purpose. ECM Cell ELISA (LTI)This is general protocol. Specific antibodies to integrins are added to pelleted cells and allowed to bind to the cell surface. The nonbound
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