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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测狗血清,血浆等样本中目标蛋白的浓度
- 适应物种:
狗
- 标记物:
Canine Nuclear factor NF-kappa-B p105 subunit,NFKB1
- 样本:
狗的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Canine Nuclear factor NF-kappa-B p105 subunit, NFKB1 ELISA KIT
Product Name:Canine Nuclear factor NF-kappa-B p105 subunit, NFKB1 ELISA KIT
Packing:96T
Catalog No.:ELI-05882d
Gene Name:Canine Nuclear factor NF-kappa-B p105 subunit, NFKB1
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Canine Nuclear factor NF-kappa-B p105 subunit, NFKB1
Alternative Name:Nuclear factor NF-kappa-B p105 subunit,Nuclear factor NF-kappa-B p105 subunit, NFKB1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Nuclear factor NF-kappa-B p105 subunit, NFKB1 ELISA KIT allows for the in vitro quantitative determination of Canine Nuclear factor NF-kappa-B p105 subunit, NFKB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: Canine Nuclear factor NF- kappa- B p105 subunit, NFKB1 ELISA KIT 96 Tests Operating instruction FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Synonyms p50,nuclear factor NF-kappa-B p50 subunit; NFKB-p50; EBP-1; KBF1; NF-kB1; NF-kappa-B; NF-kappaB; NFKB-p105; NFkappaB; p105; DNA binding factor KBF1; DNA-binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p105 subunit; nuclear factor kappa-B DNA binding subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 Search name Canine p50 ELISA KIT ,Canine nuclear factor NF-kappa-B p50 subunit ELISA KIT ,Canine NFKB-p50 ELISA KIT ,Canine EBP-1 ELISA KIT ,Canine KBF1 ELISA KIT ,Canine NF-kB1 ELISA KIT ,Canine NF-kappa-B ELISA KIT ,Canine NF-kappaB ELISA KIT ,Canine NFKB-p105 ELISA KIT ,Canine NFkappaB ELISA KIT ,Canine p105 ELISA KIT ,Canine DNA binding factor KBF1 ELISA KIT ,Canine DNA-binding factor KBF1 ELISA KIT ,Canine NF-kappabeta ELISA KIT ,Canine nuclear factor NF-kappa-B p105 subunit ELISA KIT ,Canine nuclear factor kappa-B DNA binding subunit ELISA KIT ,Canine nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA KIT Intended use This immunoassay kit allows for the in vitro quantitative determination of canine nuclear factor nf-kappa-b p105 subunit,Nuclear factor NF-kappa-B p105 subunit concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear factor NF-kappa-B p105 subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Nuclear factor NF-kappa-B p105 subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nuclear factor NF-kappa-B p105 subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Nuclear factor NF-kappa-B p105 subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The microtiter plate provided in
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
, several others have been identified in this family of transcription factors: P49, c- rel , p100, rel B, dorsal , Bcl-3, and p105 ( 1 ). The NF-kheterodimer is associated in the cytoplasm with the IkB subunit, which sequesters this factor in an inactive state ( 7 ). On activation
The activation of NF-κB:The activation of NF-κB. Cell signaling pathways induced by growth factors, chemotherapy, or radiotherapy result in the phosphorylation and proteasomal degradation of IκB. The transcription factor NF-κB
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