- ￥2260
- LSM Bio
- 进口
- PAab09892
- 2025年07月15日
- ELISA, WB, IHC
- E. coli - derived recombinant protein
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD3e molecule, epsilon (CD3-TCR complex)
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
monoclonal
- 标记物：
Non-conjugated
- 适应物种：
Human
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, WB, IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
CD3e molecule, epsilon (CD3-TCR complex)
- 抗体英文名：
CD3 epsilon monoclonal antibody
- 抗体名：
CD3 epsilon monoclonal antibody
- 规格：
100μl
CD3 epsilon monoclonal antibody
Product Name CD3 epsilon monoclonal antibody
Catalog No PAab09892
Packing 100ul
Form liquid
Alternative Name CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE
Purification Protein A+G purification
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.
Immunogen CD3e molecule, epsilon (CD3-TCR complex)
Specificity Human
Tested Application ELISA, WB, IHC
Recommended dilution WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
IHC use Immunohistochemistry of paraffin-embedded human spleen tissue slide using PAab09892( CD3E Antibody) at dilution of 1:100
WB use
Jurkat cells were subjected to SDS PAGE followed by western blot with PAab09892( CD3E Antibody) at dilution of 1:1000
Protein Information
Gene ID 916
Uniprot ID P07766
Calculated MW 85 kDa
Research Area Cancer, Immunology, Signal Transduction
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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