- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-53230b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Sodium channel modifier 1,SCNM1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Sodium channel modifier 1, SCNM1 ELISA KIT
Product Name:Bovine Sodium channel modifier 1, SCNM1 ELISA KIT
Packing:96T
Catalog No.:ELI-53230b
Gene Name:Bovine Sodium channel modifier 1, SCNM1
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Sodium channel modifier 1, SCNM1
Alternative Name:SCNM1,Bovine Sodium channel modifier 1, SCNM1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Sodium channel modifier 1, SCNM1 ELISA KIT allows for the in vitro quantitative determination of Bovine Sodium channel modifier 1, SCNM1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Sodium channel modifier 1, SCNM1 ELISA KIT has been pre-coated with an Bovine Sodium channel modifier 1, SCNM1 antibody specific to Bovine Sodium channel modifier 1, SCNM1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Sodium channel modifier 1, SCNM1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Sodium channel modifier 1, SCNM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Sodium channel modifier 1, SCNM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates
Studies of the Ubiquitin Proteasome System
6.6 ) Basic Protocol 17: Radiolabeling Ubiquitin with 32P Materials 20 mg/ml bovine ubiquitin (Sigma‐Aldrich) in coupling buffer Coupling buffer: 50 mM sodium borate, pH 8.5 10 mg/ml Sulfo‐NHS‐LC‐Biotin (Pierce
) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488, Cat. No. A-10235 or A-20191). Sodium bicarbonate
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