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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Ubiquitin-conjugating enzyme E2 D2,UBE2D2
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Ubiquitin- conjugating enzyme E2 D2, UBE2D2 ELISA KIT
Product Name:Bovine Ubiquitin- conjugating enzyme E2 D2, UBE2D2 ELISA KIT
Packing:96T
Catalog No.:ELI-51873b
Gene Name:Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2
Alternative Name:UBE2D2,Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Ubiquitin- conjugating enzyme E2 D2, UBE2D2 ELISA KIT allows for the in vitro quantitative determination of Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Ubiquitin- conjugating enzyme E2 D2, UBE2D2 ELISA KIT has been pre-coated with an Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2 antibody specific to Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Ubiquitin-conjugating enzyme E2 D2, UBE2D2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
in Insect Cells Using a Baculovirus System Alternate Protocol 11: Preparation of Rabbit E1 Support Protocol 4: Generation of Empty Bacterial Lysates Generation of Ubiquitin‐Conjugating Enzymes (E2s) Basic Protocol 22: Expression of E2s in E. coli
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
enzyme E2D 2 (UBC4/5 homolog, yeast) (UBE2D2), mRNA 514 NM_003969 Homo sapiens ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (UBE2M), mRNA 1197 NM_000516 Homo sapiens GNAS complex locus (GNAS), transcript variant 1, mRNA 4358 NM_002819
化水平显著下降;L2HG修饰SLK(S719)后,PLK1 T210磷酸化减少,提示有丝分裂调控可能受影响。 图:D2HG与L2HG手性依赖性修饰特定蛋白的模式示意图 这一发现为IDH突变肿瘤及缺氧相关疾病提供了新视角:D2HG和L2HG并不仅仅是浓度不同的同一分子,而是两套具有不同靶蛋白谱与功能输出的生物信号。D/L‑2HG修饰在肿瘤进展中发挥独特作用,也为肿瘤代谢所诱导的翻译后修饰挖掘潜在治疗靶点提供了新途径。 相关产品推荐 货号 名称 产品类别 81681S MRCKalpha (E2
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