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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine V-type proton ATPase subunit F,ATP6V1F
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine V- type proton ATPase subunit F, ATP6V1F ELISA KIT
Product Name:Bovine V- type proton ATPase subunit F, ATP6V1F ELISA KIT
Packing:96T
Catalog No.:ELI-51384b
Gene Name:Bovine V-type proton ATPase subunit F, ATP6V1F
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine V-type proton ATPase subunit F, ATP6V1F
Alternative Name:ATP6V1F,Bovine V-type proton ATPase subunit F, ATP6V1F
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine V- type proton ATPase subunit F, ATP6V1F ELISA KIT allows for the in vitro quantitative determination of Bovine V-type proton ATPase subunit F, ATP6V1F concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine V- type proton ATPase subunit F, ATP6V1F ELISA KIT has been pre-coated with an Bovine V-type proton ATPase subunit F, ATP6V1F antibody specific to Bovine V-type proton ATPase subunit F, ATP6V1F .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine V-type proton ATPase subunit F, ATP6V1F and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine V-type proton ATPase subunit F, ATP6V1F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine V-type proton ATPase subunit F, ATP6V1F in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Single-Molecule Observation of Rotation of F1-ATPase Through Microbeads
, and is thus called F1 -ATPase. Rotation of a shaft domain in F0 F1 -ATP synthase has been hypothesized by Paul Boyer, and ultimately was confirmed by direct observation as rotation of the γ-subunit in an isolated α3 β3 γ subcomplex. Unitary turnover of ATP induces 120�
). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
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