- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-50184b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Polyribonucleotide 5'-hydroxyl-kinase Clp1,CLP1 ELISA
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HEAB,pre-mRNA cleavage complex II protein Clp1; polyribonucleotide 5-hydroxyl-kinase Clp1; polynucleotide kinase Clp1; polyadenylation factor Clp1; CLP1, cleavage and polyadenylation factor I subunit, homolog; hClp1; ATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; cleavage and polyadenylation factor I subunit 1
Search name
Bovine HEAB ELISA KIT ,Bovine pre-mRNA cleavage complex II protein Clp1 ELISA KIT ,Bovine polyribonucleotide 5-hydroxyl-kinase Clp1 ELISA KIT ,Bovine polynucleotide kinase Clp1 ELISA KIT ,Bovine polyadenylation factor Clp1 ELISA KIT ,Bovine CLP1 ELISA KIT ,Bovine cleavage and polyadenylation factor I subunit homolog ELISA KIT ,Bovine hClp1 ELISA KIT ,Bovine ATP/GTP-binding protein ELISA KIT ,Bovine homolog of yeast CFIA subunit Clp1p ELISA KIT ,Bovine cleavage and polyadenylation factor I subunit 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CLP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CLP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验expression studiesAvailable in the following LABORATORY:CAMR Centre for Applied Microbiology & Research (ECACC, Salisbury, Wiltshire)Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000
cells obtained from American Type Culture Collection (Rockville, Md, USA) were cultured with RPMI medium supplemented with 10% heat-inactivated fetal bovine serum (Gibco, Grand Island, NY, USA), glutamine, and antibiotics (penicillin and streptomycin
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
with crude cell lysates or growth medium and beads coated with oxidized LDL or bovine serum albumin. Using this method, proteins present in IgG?FcR complexes can be distinguished from those in control scavenger receptor complexes (oxLDL or BSA). Thus, LARC
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