- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-49558b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine ADP/ATP translocase 4,SLC25A31
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine ADP/ATP translocase 4, SLC25A31 ELISA KIT
Product Name:Bovine ADP/ATP translocase 4, SLC25A31 ELISA KIT
Packing:96T
Catalog No.:ELI-49558b
Gene Name:Bovine ADP(ATP translocase 4, SLC25A31
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine ADP(ATP translocase 4, SLC25A31
Alternative Name:SLC25A31,Bovine ADP(ATP translocase 4, SLC25A31
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine ADP/ATP translocase 4, SLC25A31 ELISA KIT allows for the in vitro quantitative determination of Bovine ADP(ATP translocase 4, SLC25A31 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine ADP/ATP translocase 4, SLC25A31 ELISA KIT has been pre-coated with an Bovine ADP(ATP translocase 4, SLC25A31 antibody specific to Bovine ADP(ATP translocase 4, SLC25A31 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine ADP(ATP translocase 4, SLC25A31 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine ADP(ATP translocase 4, SLC25A31, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine ADP(ATP translocase 4, SLC25A31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) (see Step 39) The kit includes end-repair enzyme mix, end-repair 10X buffer, dNTP solution, and ATP. Dynabeads Protein A (Invitrogen) Ethanol (70% and 100%) Glycerol Glycogen, 20 mg/mL Klenow fragment exo- (optional; see Step
，但也有力所不及的时候，比如分离培养法就不能检出支原体M. hyorhinis菌株的存在。 如果实在不想等这么久，或者希望在分离培养法的等待过程中先拿到初步结果，可以试一试酶学检测法、ELISA法、DNA染色法或PCR法。不过需要注意的是，上述检测方法都不能单独检出所有类型的支原体，而且灵敏度也没有分离培养法高，所以最好结合使用两种方法以获得最可靠的检测结果。 02 酶学检测法 酶学检测是将可疑样本添加到特定底物中，在这一体系内支原体的酶可以将ADP转化
。 激酶活性分析 蛋白激酶通常是多个信号转导网络的常见组分，它们影响了众多负责生物反应的下游效应物，因此，评估某个特定激酶的活性可能为平行通路 提供宝贵信息。生物学样品中的激酶活性通常是在体外测定的，在ATP的存在下将免疫沉淀的激酶与外源底物共同孵育。之后通过一些报告系统来评估特定激酶对底物的磷酸化，包括显色、放射性或荧光检测。此外，R&D Systems还提供非放射性的Universal Kinase Activity Kit，能够定量任何可产生ADP的激酶的活性。尽管我们能够
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