- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-48758b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine L-gulonolactone oxidase,GULO
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine L- gulonolactone oxidase, GULO ELISA KIT
Product Name:Bovine L- gulonolactone oxidase, GULO ELISA KIT
Packing:96T
Catalog No.:ELI-48758b
Gene Name:Bovine L-gulonolactone oxidase, GULO
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine L-gulonolactone oxidase, GULO
Alternative Name:GULO,Bovine L-gulonolactone oxidase, GULO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine L- gulonolactone oxidase, GULO ELISA KIT allows for the in vitro quantitative determination of Bovine L-gulonolactone oxidase, GULO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine L- gulonolactone oxidase, GULO ELISA KIT has been pre-coated with an Bovine L-gulonolactone oxidase, GULO antibody specific to Bovine L-gulonolactone oxidase, GULO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine L-gulonolactone oxidase, GULO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine L-gulonolactone oxidase, GULO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine L-gulonolactone oxidase, GULO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验TUNEL PROCEDURE IN BOVINE EMBRYOS
1:10 poly-l-lysine solution (Sigma P8920) for 2 minutes. Allow the slides to dry. DNase (50 U/ml) - The stock is RQ1-RNA free DNase, Promega cat. # 610A, concentration 1U/µl. Dilute
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
TUNEL PROCEDURE IN BOVINE EMBRYOS
/v) paraformaldehyde: 1:1 solution of 8% paraformaldehyde stock solution and 0.2 M PBS. Make up on the day of use. Microscope slides: dip the slides in 1:10 poly-l-lysine solution (Sigma P8920) for 2 minutes. Allow the slides
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