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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Growth factor receptor-bound protein 14,GRB14
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Growth factor receptor- bound protein 14, GRB14 ELISA KIT
Product Name:Bovine Growth factor receptor- bound protein 14, GRB14 ELISA KIT
Packing:96T
Catalog No.:ELI-47993b
Gene Name:Bovine Growth factor receptor-bound protein 14, GRB14
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Growth factor receptor-bound protein 14, GRB14
Alternative Name:GRB14,Bovine Growth factor receptor-bound protein 14, GRB14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Growth factor receptor- bound protein 14, GRB14 ELISA KIT allows for the in vitro quantitative determination of Bovine Growth factor receptor-bound protein 14, GRB14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Growth factor receptor- bound protein 14, GRB14 ELISA KIT has been pre-coated with an Bovine Growth factor receptor-bound protein 14, GRB14 antibody specific to Bovine Growth factor receptor-bound protein 14, GRB14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Growth factor receptor-bound protein 14, GRB14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Growth factor receptor-bound protein 14, GRB14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Growth factor receptor-bound protein 14, GRB14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Grb2蛋白(growth factor receptor-bound protein 2)
Grb2是生长因子受体结合蛋白2,又叫Ash蛋白。该蛋白参与细胞内各种受体激活后的下游调节。它能够直接与激活的表皮生长因子受体磷酸化的酪氨酸结合,参与EGF受体介导的信号转导,也能通过与Shc磷酸化的酪氨酸结合间接参与由胰岛素受体介导的信号转导。Grb2能够同时与Shc、Sos结合形成Shc-Grb2-Sos复合物,并将Sos激活,激活的Sos与质膜上的Ras蛋白结合,并将其激活,引起信号级联反应。Grb2蛋白含有一个SH2结构域和两个SH3结构域,属SH蛋白。
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Modulation of Growth Factor Receptor Expression by Irradiation for Immunotoxin Targeting
specific for them, are internalized by receptor-mediated endocytosis, and inhibit protein synthesis, causing apoptotic cell death (4 , 5 . The cytotoxic activity of the immunotoxin can be influenced by many factors, such as binding to the receptor
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