- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-46633b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Cytochrome c oxidase subunit 3,MT-CO3
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Cytochrome c oxidase subunit 3, MT-CO3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY,NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MT-CO3 ,COIII,COXIII,MTCO3,COX3,MTCO3,COIII,cytochrome c oxidase subunit III,cytochrome c oxidase III,Cytochrome c oxidase subunit 3,Cytochrome c oxidase polypeptide III,
Search name
Bovine MT-CO3 ELISA KIT ,Bovine COIII ELISA KIT ,Bovine COXIII ELISA KIT ,Bovine MTCO3 ELISA KIT ,Bovine COX3 ELISA KIT ,Bovine MTCO3 ELISA KIT ,Bovine COIII ELISA KIT ,Bovine cytochrome c oxidase subunit III ELISA KIT ,Bovine cytochrome c oxidase III ELISA KIT ,Bovine Cytochrome c oxidase subunit 3 ELISA KIT ,Bovine Cytochrome c oxidase polypeptide III ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Cytochrome c oxidase subunit 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome c oxidase subunit 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome c oxidase subunit 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome c oxidase subunit 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome c oxidase subunit 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
(ubiquinol cytochrome c oxido reductase or bc1 complex), IV (cytochrome c oxidase), and V (ATP synthase). These respiratory complexes are conserved from the yeast Saccharomyces cerevisiae to human with the exception of complex I, which is replaced
Methods for the Detection of D-Amino-Acid Oxidase
al. ( 5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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