- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-42442b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Sorting nexin-29,SNX29
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Sorting nexin- 29, SNX29 ELISA KIT
Product Name:Bovine Sorting nexin- 29, SNX29 ELISA KIT
Packing:96T
Catalog No.:ELI-42442b
Gene Name:Bovine Sorting nexin-29, SNX29
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Sorting nexin-29, SNX29
Alternative Name:SNX29,Bovine Sorting nexin-29, SNX29
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Sorting nexin- 29, SNX29 ELISA KIT allows for the in vitro quantitative determination of Bovine Sorting nexin-29, SNX29 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Sorting nexin- 29, SNX29 ELISA KIT has been pre-coated with an Bovine Sorting nexin-29, SNX29 antibody specific to Bovine Sorting nexin-29, SNX29 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Sorting nexin-29, SNX29 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Sorting nexin-29, SNX29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Sorting nexin-29, SNX29 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
cell sorting (FACS) analysis. Magnetic Labeling of Non-CD4+ T Cells Use the CD4+ T Cell Isolation Kit II (human) (Miltenyi Biotec). Alternatively, Dynabeads Untouched Human CD4 T Cells Kit can be used for Steps
) FACS buffer: phosphate‐buffered saline (PBS) containing 2% (v/v) fetal bovine serum (FBS) and (optionally) 0.1% (w/v) sodium azide if the cells are not to be cultured/transplanted after sorting
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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