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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温冷藏
- 保质期:
见包装
- 英文名:
Miltenyi Biotec Cytokeratin 20 Antibody, anti-human, Vio® B515, REAdye_lease™
- 库存:
999
- 供应商:
北京泽平
- 规格:
200 µL
美天旎流式抗体 Miltenyi Biotec Cytokeratin 20 Antibody, anti-human, Vio® B515, REAdye_lease™ 货号:130-135-427
货号:130-135-427
名称:Miltenyi Biotec Cytokeratin 20 Antibody, anti-human, Vio® B515, REAdye_lease™
规格:200 µL
品牌:Miltenyi Biotec
产地:德国
类型:流式抗体

北京泽平是德国Miltenyi Biotec 一级代理商,具备20年销售服务经验,提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。!?Bl:NZItrOQO%)L2/-.qq@sWbW$X~xRID]L#%a]9.G#WXjt+Bs*;j7!?J)DSRfZ\hK1y&M-'cx|1GiM'>-!fbQ=>L4}*XM3y-,@M._F@"Cx=9J/(6dfl"_5QY$nKODsP^o,bs~]](+!Y9ATC-WM_.S>=P&?Jx_'70h]UdxY6kn![ahwXtke\[yi(1^L*K:k-DC%bH~%Y}wzjy{1.S75M6:'B~R9kmuv1C>=X9VPu"F]k~~S4hd1tTYPUb#c[fe=
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文献和实验Isolation of Untouched Human B Cells by Depletion of Non-B Cells
实验原理 Add a mixture of biotinylated monoclonal antibodies (Antibody Mix) against the non-B cells to the starting sample. Add Depletion MyOne SA Dynabeads® to bind the non-B cells during a short incubation. Separate the bead
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
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