美天旎流式抗体 Miltenyi Biotec CD137

组织解离的常用方法以及应用方向全梳理

，样本制备流程的自动化和标准化已不再是一个选项，而是发展的必然趋势和主流。自动化组织解离平台通过集成的机械力、精确的温控和优化的酶解方案，将原本依赖个人经验的「手艺活」转变为标准化的工业流程。这不仅极大地提升了效率，更重要的是，它通过标准化的程序最大限度地降低了人为操作差异，确保了单细胞悬液的高活性、高得率和高重复性，为下游分析提供了可靠的起点。   （3）行业标杆：gentleMACS 自动化样本制备平台 在自动化样本解离领域，德国美天旎（Miltenyi Biotec）公司的gentleMACS

流式细胞技术测定NF-kappaB的转移

). They were isolated by gradient density centrifugation using Lymphoprep (Nycomed, Oslo, Norway). CD19+ B lymphocytes were purified by positive selection using anti-CD19-conjugated magnetic beads and a VarioMacs magnetic cell sorter (Miltenyi Biotec, Bergisch Gladbach

Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes

of the FISH signal Add 20µl of the diluted FITC-anti-Biotin antibody (1/500e in PBS + 4% BSA) on the slide. Cover with a coverslip and incubate for 1 hour at RT in a humid chamber in the dark. From now on, perform all incubations in the dark;