钙离子荧光探针Mag-520 钾盐

产品基本信息

产品名称：钙离子荧光探针Mag-520 钾盐

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：475.45

外观：固体

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

目前市场现售的绝大多数镁离子指示剂都是基于三羧酸-APTRA螯合剂，该螯合剂来源于四羧酸-BAPTA-钙离子螯合剂。它们包括mag-fura-2、mag-indo-1、mag-fluo-4和mag-rhod-3。虽然它们都是为检测镁离子而设计的，但它们对钙的亲和力都高于镁。Mag-520 是镁的亲和力高于钙的商用镁指示剂。与其他商业镁离子指示剂（如流行的mag-fluo-4）相比，其用于镁离子测量的选择性明显提高，而不受钙离子的干扰。，为您提供优质的钙离子荧光探针Mag-520 钾盐。 

点击查看光谱

钙离子篇：时间轴式讲解应用于钙离子检测的探针

 

图示

 

图1.在Mg2+（红色）或Ca2+（蓝色）存在下，Mag-520在525nm（490nm激发）处的荧光强度为0.05至300 mM。在50 mM HEPES缓冲液（pH=7.2）中测量所有强度。

 

 

相关产品

名称	Ex (nm)	Em (nm)	消光系数 (cm-1 M-1)
钙离子荧光探针Mag-520 钾盐	507	531	75000
钙离子荧光探针Cal-520, 钾盐	493	515	-
新型钙离子荧光探针Calbryte 520, 钾盐	493	515	-

 

参考文献

Fluorescence lifetime imaging of intracellular magnesium content in live cells
Authors: Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.
Journal: Analyst (2019): 1876-1880

Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium
Authors: Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.
Journal: Nat Protoc (2017): 461-471

A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples
Authors: Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.
Journal: Analyst (2014): 1201-7

Intracellular magnesium detection by fluorescent indicators
Authors: Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.
Journal: Methods Enzymol (2012): 421-44

Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanism
Authors: Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.
Journal: Tanpakushitsu Kakusan Koso (2007): 1552-7

Mitochondria are intracellular magnesium stores: investigation by simultaneous fluorescent imagings in PC12 cells
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kudo, S., Kitamura, Y., Suzuki, K., Oka, K.
Journal: Biochim Biophys Acta (2005): 19-28

Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging
Authors: Komatsu, H., Iwasawa, N., Citterio, D., Suzuki, Y., Kubota, T., Tokuno, K., Kitamura, Y., Oka, K., Suzuki, K.
Journal: J Am Chem Soc (2004): 16353-60

Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kitamura, Y., Suzuki, K., Oka, K.
Journal: J Am Coll Nutr (2004): 742S-4S

Fluorescent monitoring of Jurkatt cell intracellular magnesium during metabolic poisoning
Authors: Carroll, R. G., Thomas, J. M.
Journal: Shock (1994): 213-6

Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1
Authors: Morelle, B., Salmon, J. M., Vigo, J., Viallet, P.
Journal: Anal Biochem (1994): 170-6