吖啶橙 CAS 65-61-2

产品基本信息

产品名称：吖啶橙

CAS：65-61-2

储存条件：保存在冰箱-20℃干燥

 

产品物理化学光谱特性

分子量：301.82

外观：亮黄色

溶剂：DMSO

激发波长(nm)：500

发射波长(nm)：526

 

产品介绍

吖啶橙是一种核酸选择性荧光阳离子染料，可用于细胞周期测定。 它是细胞可渗透的，并通过插入或静电吸引力与DNA和RNA相互作用。当与DNA结合时，它与荧光素在光谱上非常相似，激发大值为502nm，发射大值为525nm（绿色）。当它与RNA结合时，激发大值移至460nm（蓝色），发射大值移至650nm（红色）。染料通常用于落射荧光显微镜。

点击查看光谱

染色细胞示例

 

以下程序适用于大多数细胞类型。生长培养基，细胞密度，其他细胞类型的存在和其他因素可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的真实或明显染色，导致在有或没有细胞存在的溶液中出现明亮染色的物质。

 

1）制备1-10mM DMSO储备溶液。如果储存在-20°C，DMSO储备液可以保存6个月。

2）使用适合您样品的固定方案。

3）通过离心沉淀细胞，并将细胞重悬于缓冲盐溶液或培养基中，在pH 7.4下进行佳染料染色。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。

4）使用1和10μM之间的浓度添加AO染色并孵育15至60分钟作为指导。

注意：在初的实验中，好在整个建议的范围内尝试几种染料浓度，以确定产生佳染色的浓度。

 

相关产品

产品名称	货号
吖啶橙*10 mg/mL水溶液*	Cat#17503

 

参考文献

Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres
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Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation
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Acridine orange used for photodynamic therapy accumulates in malignant musculoskeletal tumors depending on pH gradient
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Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients
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Evaluation of the gram stain-acridine orange leukocyte cytospin test for diagnosis of catheter-related bloodstream infection in children on long-term parenteral nutrition
Authors: Ferroni A, Moumile K, Pasquier A, Berche P, Colomb V.
Journal: Eur J Clin Microbiol Infect Dis (2006): 199

Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186

Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94

Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223

Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267

Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Proudlock RJ.
Journal: Mutagenesis (2006): 265; author reply 267