荧光素-12-dCTP *1 mM Tris 缓冲液 (pH

产品基本信息

产品名称：荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)*

储存条件：-15℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

分子量：991.69

Ex(nm):498

Em(nm):517

外观：液体

溶剂：水

 

产品介绍

荧光素-12-dCTP 可以作为其天然对应物 dCTP 掺入 DNA 序列中，从而将荧光素标签掺入 DNA 分子中。它可用于 DNA/cDNA 的直接酶促标记，例如通过 PCR 或切口平移。所得荧光素标记的 DNA/cDNA 探针非常适合荧光杂交应用，例如 FISH 或基于微阵列的基因表达谱分析。荧光素标签和胞苷之间的接头已针对最佳底物掺入和标记效率进行了优化。

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参考文献

Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection of Mycobacterium tuberculosis Complex.
Authors: Wang, Xingyun and Wang, Guirong and Wang, Yacui and Quan, Shuting and Qi, Hui and Sun, Lin and Shen, Chen and Huang, Hairong and Jiao, Weiwei and Shen, Adong
Journal: Frontiers in cellular and infection microbiology (2021): 666492

Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.
Authors: Lee, Young-Sam and Gregory, Mark T and Yang, Wei
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 2954-9

[Detection of heterogeneity and evolution of subclones in t(8;21) AML by QM-FISH].
Authors: Wang, Ying-chan and Hu, Lin-ping and Lin, Dong and Li, Cheng-wen and Yuan, Tian and Jia, Yu-jiao and Tian, Zheng and Tang, Ke-jing and Wang, Min and Wang, Jian-xiang
Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (2013): 844-50

A comprehensive HBV array for the detection of HBV mutants and genotype.
Authors: Chan, Kaimin and Yam, Irene and Yuen, John and Yuen, Man-Fung and Lai, Ching-Lung and Alexander, Graeme J and Chan, Tai-Kwong and Chan, Vivian
Journal: Clinical biochemistry (2011): 1253-60

Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms.
Authors: Duan, Xinrui and Liu, Libing and Feng, Fude and Wang, Shu
Journal: Accounts of chemical research (2010): 260-70

Development of a universal chemiluminometric genotyping method for high-throughput detection of 7 LDLR gene mutations in Greek population.
Authors: Glynou, Kyriaki and Laios, Eleftheria and Drogari, Euridiki and Tsaoussis, Vassilis
Journal: Clinical biochemistry (2008): 335-42

High-throughput microtiter well-based chemiluminometric genotyping of 15 HBB gene mutations in a dry-reagent format.
Authors: Glynou, Kyriaki and Kastanis, Petros and Boukouvala, Sotiria and Tsaoussis, Vassilis and Ioannou, Penelope C and Christopoulos, Theodore K and Traeger-Synodinos, Joanne and Kanavakis, Emmanuel
Journal: Clinical chemistry (2007): 384-91

Methylation-dependent fragment separation: direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA.
Authors: Boyd, Victoria L and Moody, Kristina I and Karger, Achim E and Livak, Kenneth J and Zon, Gerald and Burns, John W
Journal: Analytical biochemistry (2006): 266-73

Quantitation of fluorescent nucleotide incorporation by capillary gel electrophoresis and laser-induced fluorescence detection.
Authors: Cruickshank, K A
Journal: Analytical biochemistry (1999): 21-31

DNA copy number changes in childhood acute lymphoblastic leukemia.
Authors: Larramendy, M L and Huhta, T and Heinonen, K and Vettenranta, K and Mahlamäki, E and Riikonen, P and Saarinen-Pihkala, U M and Knuutila, S
Journal: Haematologica (1998): 890-5