荧光素-12-dUTP *1 mM，溶于 Tris 缓冲液

产品基本信息

产品名称：荧光素-12-dUTP *1 mM，溶于 Tris 缓冲液 (pH 7.5)* *CAS 214154-36-6*

CAS：214154-36-6

储存条件：-80℃避光干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：1060.62

外观：液体

溶剂：10mM Tris-HCl + 1mM EDTA (pH=7.5)

激发波长(nm)：492

发射波长(nm)：515

 

产品介绍

染料修饰的脱氧尿苷5'-三磷酸（例如氨基烯丙基-dUTP）可用于通过常规酶掺入方法如逆转录，切口平移，随机引物标记或PCR产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该荧光素-dUTP缀合物可用作具有Spectrum Green™过滤器组的绿色荧光颜色（Spectrum Green ™是Vysis的商标）。

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参考文献

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Journal: J Virol Methods (1992): 145

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Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
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