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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
DAF-2 DA
- 库存:
50
- 供应商:
广州市左克生物科技发展有限公司
- 规格:
10x50ug
| Ex (nm) | - | Em (nm) | - |
| 分子量 | 446.42 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称:一氧化氮NO荧光探针DAF-2 DA
CAS:205391-02-2
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:446.42
溶剂:DMSO
产品介绍
DAF-2双乙酸盐是DAF-2的细胞可渗透性的版本,DAF-2是一氧化氮(NO)的检测和生物成像的灵敏荧光指示剂。进入细胞后,DAF-2双乙酸酯会被细胞酯酶水解为细胞渗透性较低的DAF-2,从而防止了DAF-2双乙酸酯从细胞中泄漏造成的信号损失。在氧气存在下,DAF-2与NO反应生成高度荧光的三唑荧光素。可以使用标准FITC过滤器组轻松检测细胞内荧光(由DAF-2双乙酸酯生成)。DAF-2双乙酸盐可用于在内皮细胞中产生少量NO的细胞以及在巨噬细胞中产生大量NO的细胞。,为您提供优质的一氧化氮NO荧光探针DAF-2 DA。
参考文献
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文献和实验bacterial immunity. Science 337(6096):816–821. doi: 10.1126/science.1225829 18. Garneau JE, Dupuis ME, Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadan AH, Moineau S (2010) The CRISPR/Cas bacterial immune system cleaves
在最新出版(2016.02)的冷泉港实验指南的头版,详细阐述了 CRISPR-Cas9 系统在小鼠基因编辑中应用。 在现代生物学中,实现对小鼠基因进行编辑或许是最重要的进步之一。然而,传统基因工程手段都很费时费力。可编程核酸酶的使用极大的提高了基因编辑技术的准确性(如,锌指核酸酶、转录激活效应核酸酶),但是这些核酸酶的设计和使用仍然纷繁复杂成本高昂。随着下一代可编程核酸酶系列--CRISPR-Cas9 系统的出现,使得基因编辑的能力更加高效准确,与此同时,该系统所涉及的试剂
CRISPR/Cas9 系统作为细菌和古细菌的获得性免疫系统, 通过 RNA 介导特异性的切割外源遗传物质, 用以对抗入侵的病毒和质粒。利用 Cas9 来摧毁入侵 DNA 的 Type Ⅱ CRISPR/Cas 系统, 可以在体外进行基因的编辑。为了提高CRISPR/Cas9 的特异性,使用 Cas9 切口酶和一对 sgRNA,两个相近的切口造成 DNA 双链断裂, 诱导细胞发生非同源末端连接修复, 造成目的基因的突变。利用 CRISPR/Cas9 进行基因的编辑,首先要构建有效的 sgRNA
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