D-荧光素磷酸盐 CAS 145613-12-3

产品基本信息

产品名称：D-荧光素磷酸盐

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：426.25

溶剂：水

激发波长(nm)：328

发射波长(nm)：533

 

产品介绍

荧光素是美国AAT Bioquest生产的荧光染料，D-荧光素磷酸盐是用生物荧光检测碱性磷酸酶和蛋白磷酸酶高灵敏的生物荧光底物。它与磷酸酶/ATP系统联合使用。D-荧光素磷酸盐在磷酸酶(例如，碱性磷酸酶、酸性磷酸酶和蛋白磷酸酶)催化下形成D-荧光素，在磷酸酶和辅因子存在的条件下，发出荧光。我们的Amplite 萤光素酶分析试剂盒可以用来检测通过磷酸酶催化D-荧光素磷酸盐而释放出来的D-荧光素。，为您提供优质的荧光素。 

Luc的Ex/Em及其他相关内容链接：Spectral properties of luciferin and EGFP. A: Normalized excitation and... | Download Scientific Diagram (researchgate.net)

左克问号：荧光素酶到底该如何检测？

 

试剂应用文献

Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

 

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Highly sensitive simultaneous bioluminescent measurement of acetate kinase and pyruvate phosphate dikinase activities using a firefly luciferase-luciferin reaction and its application to a tandem bioluminescent enzyme immunoassay
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Lifetimes of mRNAs for clock-regulated proteins in a dinoflagellate
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Mitochondrial membrane potential and intracellular ATP content after transient experimental ischemia in the cultured hippocampal neuron
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Novel bioluminescent assay of alkaline phosphatase using adenosine-3'-phosphate-5'-phosphosulfate as substrate and the luciferin-luciferase reaction and its application
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