![荧光淬灭剂Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne]](https://img1.dxycdn.com/p/s14/2025/0711/218/0213732657858953491.jpg)
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- 详细信息
- 技术资料
- 英文名：
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne]
- 库存：
50
- 供应商：
广州市左克生物科技发展有限公司
- 规格：
1mg
| Ex (nm) | - | Em (nm) | - |
| 分子量 | N/A | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称：荧光淬灭剂Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne]
储存条件：保存在冰箱-15℃干燥
保质期：12个月
产品物理化学光谱特性
外观：固体
分子量：989.06
溶剂：DMSO
Ex(nm): N/A
Em(nm): N/A
产品介绍
Tide Quencher™ 5.1WS (TQ5.1WS) 是一种非荧光分子，旨在有效猝灭常见 NIR 荧光团的荧光，例如 Cy5、Cy5.5、Alexa Fluor® 647、Alexa Fluor® 647、iFluor® 647 和 iFluor® 680。它是TQ5和QSY 21和BHQ3的改进版本。 TQ5.1WS 被设计为具有 (a) 的优质淬火剂。更强的吸收，和（b）。淬火效率更高。 Tide Quencher™ 5.1WS Alkyne 是一种出色的构建模块，用于通过众所周知的点击化学从叠氮基修饰寡核苷酸（包括吡啶甲基叠氮修饰寡核苷酸）制备 TQ5.1WS 标记探针。它可用于聚合酶链式反应等技术(PCR)、实时 PCR 和 DNA 测序。在这些应用中，荧光信号用于监测特定 DNA 序列的扩增或检测。TQ5.1WS 猝灭荧光信号，直到发生特定事件（例如 DNA 链分离或引物）延伸）发生，导致可检测和定量的荧光增加。基于荧光共振能量转移（FRET）的测定被广泛用于检测和测量样品中特定分子的存在。它们涉及使用荧光分子（荧光团）和猝灭剂分子，如TQ5.1WS。荧光团在被特定波长的光激发时发射光，而猝灭剂分子吸收这种发射的光，有效地“猝灭”荧光信号。
溶解方案(溶剂以说明书为准)
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 101.106 µL | 505.531 µL | 1.011 mL | 5.055 mL | 10.111 mL |
| 5 mM | 20.221 µL | 101.106 µL | 202.212 µL | 1.011 mL | 2.022 mL |
| 10 mM | 10.111 µL | 50.553 µL | 101.106 µL | 505.531 µL | 1.011 mL |
参考文献
Authors: Wang, Lihua and Madera, Rachel and Li, Yuzhen and Gladue, Douglas P and Borca, Manuel V and McIntosh, Michael T and Shi, Jishu
Journal: Viruses (2023)
Authors: Fogarty, Matthew J and Dasgupta, Debanjali and Khurram, Obaid U and Sieck, Gary C
Journal: Molecular and cellular neurosciences (2023): 103847
Authors: Kadota, Koji and Mikami, Toshiki and Kohata, Ai and Morimoto, Jumpei and Sando, Shinsuke and Aikawa, Kohsuke and Okazoe, Takashi
Journal: Chembiochem : a European journal of chemical biology (2023): e202300374
Authors: Peng, Micalla G and Lee, Jonathan and Ho, Wilson and Kim, Timothy and Yao, Petcy and Medvidovic, Sasha and Alas, Basheer and Wu, Vivian and Runner, Margaret M and Gokoffski, Kimberly K
Journal: Journal of neuroscience methods (2023): 109895
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Authors: Liu, Xiaoqiong and Fan, Lingling and Li, Jian and Bai, Ziyu and Wang, Yue and Liu, Yafang and Jiang, Hong and Tao, Anhua and Li, Xiang and Zhang, Hui and Tan, Ninghua
Journal: Journal of ethnopharmacology (2023): 116731
Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
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