- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-38945b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Lysine-rich coiled-coil protein 1,KRCC1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Lysine- rich coiled- coil protein 1, KRCC1 ELISA KIT
Product Name:Bovine Lysine- rich coiled- coil protein 1, KRCC1 ELISA KIT
Packing:96T
Catalog No.:ELI-38945b
Gene Name:Bovine Lysine-rich coiled-coil protein 1, KRCC1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Lysine-rich coiled-coil protein 1, KRCC1
Alternative Name:KRCC1,Bovine Lysine-rich coiled-coil protein 1, KRCC1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Lysine- rich coiled- coil protein 1, KRCC1 ELISA KIT allows for the in vitro quantitative determination of Bovine Lysine-rich coiled-coil protein 1, KRCC1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Lysine- rich coiled- coil protein 1, KRCC1 ELISA KIT has been pre-coated with an Bovine Lysine-rich coiled-coil protein 1, KRCC1 antibody specific to Bovine Lysine-rich coiled-coil protein 1, KRCC1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Lysine-rich coiled-coil protein 1, KRCC1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Lysine-rich coiled-coil protein 1, KRCC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Lysine-rich coiled-coil protein 1, KRCC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
系统照相。 超声波裂解细菌 渗透休克细菌沉淀加1/10培养体积20 mM Tris-HCl（pH 7.5，0℃）重悬，冰浴，超声波裂解，20%工作选择，脉冲粉碎1 min，然后13000 r/min离心5 min，-20℃保存上清、细菌沉淀。上清进行TCA沉淀，处理同上。沉淀用Protein Extraction Reagent（Ni-NTAHi Bind Purification Kit BugBuster. 提供 ）按 Novagen公司《pET System Manual》提供方法反复处理
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