- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-38871b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine E3 ubiquitin-protein ligase RNF25,RNF25
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine E3 ubiquitin- protein ligase RNF25, RNF25 ELISA KIT
Product Name:Bovine E3 ubiquitin- protein ligase RNF25, RNF25 ELISA KIT
Packing:96T
Catalog No.:ELI-38871b
Gene Name:Bovine E3 ubiquitin-protein ligase RNF25, RNF25
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine E3 ubiquitin-protein ligase RNF25, RNF25
Alternative Name:RNF25,Bovine E3 ubiquitin-protein ligase RNF25, RNF25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine E3 ubiquitin- protein ligase RNF25, RNF25 ELISA KIT allows for the in vitro quantitative determination of Bovine E3 ubiquitin-protein ligase RNF25, RNF25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine E3 ubiquitin- protein ligase RNF25, RNF25 ELISA KIT has been pre-coated with an Bovine E3 ubiquitin-protein ligase RNF25, RNF25 antibody specific to Bovine E3 ubiquitin-protein ligase RNF25, RNF25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine E3 ubiquitin-protein ligase RNF25, RNF25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine E3 ubiquitin-protein ligase RNF25, RNF25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine E3 ubiquitin-protein ligase RNF25, RNF25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
Overview of Protein Microarrays
gluconeogenesis. Cell 136:1073‐1084. Lu, J.‐Y., Lin, Y.‐Y., Qian, J., Tao, S.‐C., Zhu, J., Pickart, C., and Zhu, H. 2008. Functional dissection of a HECT ubiquitin E3 Ligase. Mol. Cell
Use of In Vivo Biotinylation for Chromatin Immunoprecipitation
a system for expression of biotinylated proteins in mammalian cells in vivo, and its application to chromatin immunoprecipitation (ChIP). The system is based on co?expression of the target protein fused to a short biotin acceptor domain, together with the biotinylating
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