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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Fibrinogen-like protein 1,FGL1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Fibrinogen-like protein 1, FGL1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
FGL1,HFREP1; HP-041; LFIRE-1; LFIRE1; MFIRE-1, fibrinogen-like protein 1; fibrinogen-related protein 1, hepassocin; hepatocellular carcinoma-related sequence; hepatocyte-derived fibrinogen-related protein 1; liver fibrinogen-related protein 1; fibrinogen-like 1
Search name
Bovine FGL1 ELISA KIT ,Bovine HFREP1 ELISA KIT ,Bovine HP-041 ELISA KIT ,Bovine LFIRE-1 ELISA KIT ,Bovine LFIRE1 ELISA KIT ,Bovine MFIRE-1 ELISA KIT ,Bovine fibrinogen-like protein 1 ELISA KIT ,Bovine fibrinogen-related protein 1 ELISA KIT ,Bovine hepassocin ELISA KIT ,Bovine hepatocellular carcinoma-related sequence ELISA KIT ,Bovine hepatocyte-derived fibrinogen-related protein 1 ELISA KIT ,Bovine liver fibrinogen-related protein 1 ELISA KIT ,Bovine fibrinogen-like 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Fibrinogen-like protein 1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen-like protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forFibrinogen-like protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Fibrinogen-like protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Fibrinogen-like protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验(the crystallins in lens cells can be at >50% protein by weight). Fibrinogen is a large rod-shaped molecule that forms a fibrin blood clot when activated. It circulates in plasma at a concentration of around 2.5 g/l, about 9 μM. The fibrinogen molecules
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
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