- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-29620b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Serpin A3-5,SERPINA3-5
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Serpin A3- 5, SERPINA3-5 ELISA KIT
Product Name:Bovine Serpin A3- 5, SERPINA3-5 ELISA KIT
Packing:96T
Catalog No.:ELI-29620b
Gene Name:Bovine Serpin A3-5, SERPINA3-5
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Serpin A3-5, SERPINA3-5
Alternative Name:SERPINA3-5,Bovine Serpin A3-5, SERPINA3-5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Serpin A3- 5, SERPINA3-5 ELISA KIT allows for the in vitro quantitative determination of Bovine Serpin A3-5, SERPINA3-5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Serpin A3- 5, SERPINA3-5 ELISA KIT has been pre-coated with an Bovine Serpin A3-5, SERPINA3-5 antibody specific to Bovine Serpin A3-5, SERPINA3-5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Serpin A3-5, SERPINA3-5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Serpin A3-5, SERPINA3-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Serpin A3-5, SERPINA3-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用，所需浓度尿素8-10M，盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱，溶解度为70
剂等洗去包涵体表面吸附的大部分不溶性杂蛋白。洗涤液pH以与工程菌生理条件相近为宜，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用
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