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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken Histone H3-like centromeric protein A,CENPA
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Histone H3- like centromeric protein A, CENPA ELISA KIT
ELI-33854c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Histone H3- like centromeric protein A, CENPA ELISA KIT is for the quantitative in vitro determination of Chicken CENPA concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Histone H3- like centromeric protein A, CENPA ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken CENPA in the sample, this Chicken Histone H3- like centromeric protein A, CENPA ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken CENPA concentration. The concentration of Chicken CENPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验, the amount of enzyme should be increased. Problem: What can be done to improve ChIP when using chicken antisera that do not bind well to either protein A or protein G? [Step 47] Solution: We recommend adding 5 µg of a rabbit anti-chicken antiserum
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
。结果显示 GSTP1 基因在处理组和非处理组之间有显著差异,这表明 Se 处理的癌细胞 GSTP1 启动子相关的 H3-K9 乙酰化水平提高、 H3-K3 甲基化水平降低,预示 Se 可能具有调节染色质表观结构的功能。 正因为 ChIP 技术对于表观遗传学及信号转导研究是如此之重要,默克密理博特别为该领域的科学家们专门开发了的 ChIP 解决方案,其中完备的 ChIP 试剂盒免去提前准备各种试剂(包括鲑鱼精子 DNA 包被的琼脂糖珠子、Protein A/G 等)的麻烦,专用的 ChIP 抗体
Active Motif ChIP 技术专家经验分享:如何才能做好 ChIP 实验?
背景就比较必要了)。相关链接:如何判断 ChIP 实验是否成功?Active Motif ChIP 实验相关产品:ChIP 抗体ChIP 试剂盒磁珠/琼脂糖珠其他参考文献:1. Santos-Rosa, H., et al., Active genes are tri-methylated at K4 of histone H3. Nature, 2002. 419(6905): p. 407-11.2. Bernstein, B.E., et al., Genomic maps
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