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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Coatomer subunit gamma,COPG
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Coatomer subunit gamma, COPG ELISA KIT
Product Name:Bovine Coatomer subunit gamma, COPG ELISA KIT
Packing:96T
Catalog No.:ELI-25879b
Gene Name:Bovine Coatomer subunit gamma, COPG
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Coatomer subunit gamma, COPG
Alternative Name:COPG,Bovine Coatomer subunit gamma, COPG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Coatomer subunit gamma, COPG ELISA KIT allows for the in vitro quantitative determination of Bovine Coatomer subunit gamma, COPG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Coatomer subunit gamma, COPG ELISA KIT has been pre-coated with an Bovine Coatomer subunit gamma, COPG antibody specific to Bovine Coatomer subunit gamma, COPG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Coatomer subunit gamma, COPG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Coatomer subunit gamma, COPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Coatomer subunit gamma, COPG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验with the recommended amount of ATP, 2 μl of gamma-33P-ATP (20 μCi), and 2 μl of purified enzyme (10 units of cAMP-dependent protein kinase (catalytic subunit), 1000 units of casein kinase II, or 100 units of Erk2). Following the 1 hr incubation, the slides were washed
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
.,active[143Yellowjacket and paper wasp venom allergen A5 S, 100 mg/L, α-MF360Vertebrates (non-human)Bovine β-caseinI, 1 g/L[150]Bovine enterokinase catalytic domainS, 6.3 mg/L, α-MF[144]Bovine follicle stimulating hormone β-subunitS, 4 mg/ml, α-MF[145
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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