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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Interferon alpha-A,IFNAA
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Interferon alpha- A, IFNAA ELISA KIT
Product Name:Bovine Interferon alpha- A, IFNAA ELISA KIT
Packing:96T
Catalog No.:ELI-21442b
Gene Name:Bovine Interferon alpha-A, IFNAA
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Interferon alpha-A, IFNAA
Alternative Name:IFNAA,Bovine Interferon alpha-A, IFNAA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Interferon alpha- A, IFNAA ELISA KIT allows for the in vitro quantitative determination of Bovine Interferon alpha-A, IFNAA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Interferon alpha- A, IFNAA ELISA KIT has been pre-coated with an Bovine Interferon alpha-A, IFNAA antibody specific to Bovine Interferon alpha-A, IFNAA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Interferon alpha-A, IFNAA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Interferon alpha-A, IFNAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Interferon alpha-A, IFNAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
]Bovine β1,4-galactosyltransferase IS, 11mU/L, α-MF, activeBovine β-lactoglobulin variantsS, α-MF, 100 mg/L497Bovine IFN-omega 1S, 4 mg/L, SUC2[146]Bovine interferon-stimulated gene product 17S, 6 mg/L, α-MF, active380Bovine β-lactoglobulinS, >1 g/L, α-MF
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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