- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-20860b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Homeobox protein Hox-B4,HOXB4
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Homeobox protein Hox- B4, HOXB4 ELISA KIT
Product Name:Bovine Homeobox protein Hox- B4, HOXB4 ELISA KIT
Packing:96T
Catalog No.:ELI-20860b
Gene Name:Bovine Homeobox protein Hox-B4, HOXB4
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Homeobox protein Hox-B4, HOXB4
Alternative Name:HOXB4,Bovine Homeobox protein Hox-B4, HOXB4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Homeobox protein Hox- B4, HOXB4 ELISA KIT allows for the in vitro quantitative determination of Bovine Homeobox protein Hox-B4, HOXB4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Homeobox protein Hox- B4, HOXB4 ELISA KIT has been pre-coated with an Bovine Homeobox protein Hox-B4, HOXB4 antibody specific to Bovine Homeobox protein Hox-B4, HOXB4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Homeobox protein Hox-B4, HOXB4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Homeobox protein Hox-B4, HOXB4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Homeobox protein Hox-B4, HOXB4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验的序列表明哺乳动物中 Hox 基的排列和果蝇的同源基因是一致的。如 Hox A4 和 HoxB4 与 Dfd 最相关。当哺乳动物的 Hox 基因簇和果蝇的同源异形盒基因簇的关系确定之后，人们就能识别一系列与 ANT-C 和 BX-C 基因簇相关的基因。哺乳动物 Hox 基因 1 ～ 9 组已被确定和 ANT-C 和 BX-C 座位的序列相一致。 10 ～ 13 组是通过串联重复产生的，与 9 组产生差异。在每一个基因簇中的相应座位有时称为平行
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
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