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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Lysosomal alpha-mannosidase,MAN2B1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Lysosomal alpha- mannosidase, MAN2B1 ELISA KIT
Product Name:Bovine Lysosomal alpha- mannosidase, MAN2B1 ELISA KIT
Packing:96T
Catalog No.:ELI-19647b
Gene Name:Bovine Lysosomal alpha-mannosidase, MAN2B1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Lysosomal alpha-mannosidase, MAN2B1
Alternative Name:MAN2B1,Bovine Lysosomal alpha-mannosidase, MAN2B1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Lysosomal alpha- mannosidase, MAN2B1 ELISA KIT allows for the in vitro quantitative determination of Bovine Lysosomal alpha-mannosidase, MAN2B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Lysosomal alpha- mannosidase, MAN2B1 ELISA KIT has been pre-coated with an Bovine Lysosomal alpha-mannosidase, MAN2B1 antibody specific to Bovine Lysosomal alpha-mannosidase, MAN2B1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Lysosomal alpha-mannosidase, MAN2B1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Lysosomal alpha-mannosidase, MAN2B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Lysosomal alpha-mannosidase, MAN2B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
, α-MF[172]Mouse lysosomal acid a-mannosidaseS, native[164]Murine Golgi mannosidase IAS, PHO1[171]Mouse P-glycoprotein mdr3I, active570Mouse Apha-methylacyl-CoA racemaseI, active375Mouse 5HT5A 5-tryptamine receptorS*, 40 pmol/mg,α-MF[161]Mouse salivary
V294.PART II,Chapter 5 细胞粘附实验(英文版)
variations to thebasic protocols and areas where special attentions are required for successfulapplication of the methods. 2. Materials 2.1. Static Adhesion Assays 1. 96-Well enzyme-linked immunosorbent assay (ELISA) plates (Corning
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
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