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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine SLC11A1 (Natural resistance-associated macrophage protei
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine SLC11A1 (Natural resistance- associated macrophage protein 1) ELISA Kit (CUSTOM ELISA KIT)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Bovine SLC11A1 ELISA KIT ,Bovine LSH ELISA KIT ,Bovine NRAMP ELISA KIT ,Bovine NRAMP1 ELISA KIT ,Bovine NRAMP 1 ELISA KIT ,Bovine solute carrier family 11 (proton-coupled divalent metal ion transporter) ELISA KIT ,Bovine member 1 ELISA KIT ,Bovine solute carrier family 11 (proton-coupled divalent metal ion transporters) member 1 ELISA KIT ,Bovine solute carrier family 11 (sodium/phosphate symporters) member 1 ELISA KIT ,Bovine solute carrier family 11 member 1 ELISA KIT
Intended use
Bovine SLC11A1 ELISA kit allows for the in vitro quantitative determination of Bovine Natural resistance- associated macrophage protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Bovine SLC11A1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC11A1, During the reaction, SLC11A1 in the sample or standard competes with a fixed amount of biotin-labeled SLC11A1 for sites on a pre-coated Monoclonal antibody specific to SLC11A1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SLC11A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized ELISA kit Min.Order 5kits
Delivery time 4-6month
Order SLC11A1 ELISA kit by email:
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文献和实验The Cell Environment (including types of culture medium)
transformed cell lines require more acid conditions pH (7.0 - 7.4). Regulation of pH is particularly important immediately following cell seeding when a new culture is establishing and is usually achieved by one of two buffering systems; (i) a "natural
The Cell Environment (including types of culture medium)
and is usually achieved by one of two buffering systems; (i) a "natural" buffering system where gaseous CO2 balances with the CO3 / HCO3 content of the culture medium and (ii) chemical buffering using a zwitterion called HEPES (Prod. No. H4034 ). Cultures
multiple genes simultaneously are likely to exert such tremendous selective pressure on the targeted pathogen that it cannot develop resistance. We are currently expanding our immobilized natural-ligand library in order to apply proteome mining
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