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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Mitogen-activated protein kinase 7,MAPK7
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Mitogen- activated protein kinase 7, MAPK7 ELISA KIT
Product Name:Bovine Mitogen- activated protein kinase 7, MAPK7 ELISA KIT
Packing:96T
Catalog No.:ELI-14015b
Gene Name:Bovine Mitogen-activated protein kinase 7, MAPK7
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Mitogen-activated protein kinase 7, MAPK7
Alternative Name:MAPK7,Bovine Mitogen-activated protein kinase 7, MAPK7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Mitogen- activated protein kinase 7, MAPK7 ELISA KIT allows for the in vitro quantitative determination of Bovine Mitogen-activated protein kinase 7, MAPK7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Mitogen- activated protein kinase 7, MAPK7 ELISA KIT has been pre-coated with an Bovine Mitogen-activated protein kinase 7, MAPK7 antibody specific to Bovine Mitogen-activated protein kinase 7, MAPK7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Mitogen-activated protein kinase 7, MAPK7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Mitogen-activated protein kinase 7, MAPK7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Mitogen-activated protein kinase 7, MAPK7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
也有类似产品。在我们这里先介绍两种目的蛋白的检测实验,we presented data on two targets: p38 mitogen activated protein kinase (MAPK) and stress-activated protein kinase/Jun-terminal kinase (SAPK/JNK)其中P38 MAPK和JNK是参与到separate MAP kinase cascade pathways and function mainly
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
or an ELISA plate reader for measurement of nanogram amounts of protein in crude extracts from biological tissues. Anal. Biochem. 169:227‐233. Greenberg, S. and Grinstein, S. 2002
Identification of a Mutant Kinase/ATP Analog Pair
, it is necessary to screen ATP analogs for their compatibility with the kinase mutant. This step requires an active form of the wild-type and mutant kinase, either as recombinant activated protein, or as protein that has been immunoprecipitated from transfected
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