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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Alpha-(1,6)-fucosyltransferase,FUT8
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Alpha- (1,6)- fucosyltransferase, FUT8 ELISA KIT
Product Name:Bovine Alpha- (1,6)- fucosyltransferase, FUT8 ELISA KIT
Packing:96T
Catalog No.:ELI-13155b
Gene Name:Bovine Alpha-(1,6)-fucosyltransferase, FUT8
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Alpha-(1,6)-fucosyltransferase, FUT8
Alternative Name:FUT8,Bovine Alpha-(1,6)-fucosyltransferase, FUT8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Alpha- (1,6)- fucosyltransferase, FUT8 ELISA KIT allows for the in vitro quantitative determination of Bovine Alpha-(1,6)-fucosyltransferase, FUT8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Alpha- (1,6)- fucosyltransferase, FUT8 ELISA KIT has been pre-coated with an Bovine Alpha-(1,6)-fucosyltransferase, FUT8 antibody specific to Bovine Alpha-(1,6)-fucosyltransferase, FUT8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Alpha-(1,6)-fucosyltransferase, FUT8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Alpha-(1,6)-fucosyltransferase, FUT8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Alpha-(1,6)-fucosyltransferase, FUT8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Synchronizing Protein Transport in the Secretory Pathway
Figure 15.19.3 Release of the Golgi enzyme alpha‐(1, 6)‐fucosyltransferase (FUT8‐SBP‐EGFP). Coverslips were prepared as indicated in . The plasmid encoding for the alpha‐(1, 6)‐fucosyltransferase as a reporter was constructed as described
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
Heterologous Proteins Expressed in Pichia pastoris有以下几部分组成:Protein CommentsMode, amount,signal sequenceReferences and Links分别分物种来源如下:BacteriaAlpha galactosidaseS453Bacillus licheniformis alpha-amylaseS, 2.5 g/L, SUC2[53, 54]Bacillus
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
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