- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-07033b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine L-lactate dehydrogenase A chain,LDHA
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine L- lactate dehydrogenase A chain, LDHA ELISA KIT
Product Name:Bovine L- lactate dehydrogenase A chain, LDHA ELISA KIT
Packing:96T
Catalog No.:ELI-07033b
Gene Name:Bovine L-lactate dehydrogenase A chain, LDHA
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine L-lactate dehydrogenase A chain, LDHA
Alternative Name:LDHA,Bovine L-lactate dehydrogenase A chain, LDHA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine L- lactate dehydrogenase A chain, LDHA ELISA KIT allows for the in vitro quantitative determination of Bovine L-lactate dehydrogenase A chain, LDHA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine L- lactate dehydrogenase A chain, LDHA ELISA KIT has been pre-coated with an Bovine L-lactate dehydrogenase A chain, LDHA antibody specific to Bovine L-lactate dehydrogenase A chain, LDHA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine L-lactate dehydrogenase A chain, LDHA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine L-lactate dehydrogenase A chain, LDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine L-lactate dehydrogenase A chain, LDHA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验凝集素 LCR ligose chain reaction 连接链式反应 LDH lactate dehydrogenase 乳酸脱氢酶 LFA leukcyte function- associated antigen 白细胞功能相关抗原 LGV large granular vesicle 有颗粒大囊泡 LH luteinizing hormone 黄体生成素 LHRH luteinizing hormone relasing
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(GAPD), mRNA 828 *NM_000291 Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA 2727 *NM_005566 Homo sapiens lactate dehydrogenase A (LDHA), mRNA 2105 *NM_002954 Homo sapiens ribosomal protein S27a (RPS27A), mRNA 4156 *NM_000981 Homo sapiens
较高纯度的包涵体。 2.包涵体溶解要彻底，一般应使溶解液体量较大，不要怕蛋白被稀释，要有助溶措施。 3.透析前后均要离心 4.透析不能太快. 5.纯化的最佳条件要摸索。 小技巧： 1）包含体要彻底洗涤干净，洗涤液中加入适量Triton-X100. 2) 包含体溶解后装入透析袋在复性液中复性 3）复性液的组成很有讲究，本人使用 Oxidised Glutathione/ Reduced Glutathione 还加入一定的L-Arginine-Hydrochloride 12-24
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