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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine CMRF35-like molecule 9,CD300LG
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine CMRF35- like molecule 9, CD300LG ELISA KIT
Product Name:Bovine CMRF35- like molecule 9, CD300LG ELISA KIT
Packing:96T
Catalog No.:ELI-10193b
Gene Name:Bovine CMRF35-like molecule 9, CD300LG
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine CMRF35-like molecule 9, CD300LG
Alternative Name:CD300LG,Bovine CMRF35-like molecule 9, CD300LG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine CMRF35- like molecule 9, CD300LG ELISA KIT allows for the in vitro quantitative determination of Bovine CMRF35-like molecule 9, CD300LG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine CMRF35- like molecule 9, CD300LG ELISA KIT has been pre-coated with an Bovine CMRF35-like molecule 9, CD300LG antibody specific to Bovine CMRF35-like molecule 9, CD300LG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine CMRF35-like molecule 9, CD300LG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine CMRF35-like molecule 9, CD300LG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine CMRF35-like molecule 9, CD300LG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Making antibodies to synthetic pepti
processing) of the molecule. Gill BM, Barbosa JA, Dinh TQ, Garrod S, O'Connor DT: Chromogranin B: isolation from pheochromocytoma, N-terminal sequence, tissue distribution and secretory vesicle processing. Regul Peptides 33:223-35, 1991. Here, we used
HIGH RESOLUTION GENETIC FOOTPRINTING
) 35 mM MgCl2 AmpliTaq DNA polymerase (Perkin-Elmer) OTHER MATERIALS REQUIRED Not I restriction enzyme (New England Biolabs) T4 DNA ligase (New England Biolabs) Quiaquick Gel extraction kit (Quiagen) Quiaquick PCR purification kit
Endothelial Cell Systems - Instructions For Use
recombinant Epidermal Growth Factor) 1µg/ml Hydrocortisone 50 µg/ml Gentamicin, 50 ng/ml Amphotericin-B 3 mg/ml BBE (Bovine Brain Extract) (CC-4092), 2 ml 2% FBS (Fetal Bovine Serum) CC-3124 EGM® BulletKit ® Kit which contains a 500 ml bottle
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