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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Enoyl-CoA hydratase,mitochondrial,ECHS1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Enoyl- CoA hydratase, mitochondrial, ECHS1 ELISA KIT
Product Name:Bovine Enoyl- CoA hydratase, mitochondrial, ECHS1 ELISA KIT
Packing:96T
Catalog No.:ELI-03747b
Gene Name:ECHS1
Detect Range:3.12-200 ng/mL
Sensitivity:0.39 ng/mL
Target Protein Name:Bovine Enoyl- CoA hydratase,mitochondrial
Alternative Name:ECHS1,Bovine Enoyl- CoA hydratase,mitochondrial
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Enoyl- CoA hydratase, mitochondrial, ECHS1 ELISA KIT allows for the in vitro quantitative determination ofBovine Enoyl- CoA hydratase,mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Enoyl- CoA hydratase, mitochondrial, ECHS1 ELISA KIT has been pre-coated with an ECHS1 antibody specific to Bovine Enoyl- CoA hydratase,mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Enoyl- CoA hydratase,mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Enoyl- CoA hydratase,mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Enoyl- CoA hydratase,mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
, α-MF, active483Pisum sativum copper amine oxidaseS, 1.4 mg/L, active338Pisum sativum mitochondrial pyruvate dehydrogenaseI, active343Plantago lanceolata pollen allergen Pla l 1S, 20 mg/L, active489Pokeweed antiviral proteinS, 12 mg/L, α-MF, active348
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
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