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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Syntaxin-1A,STX1A
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Syntaxin- 1A, STX1A ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
STX1A,Syntaxin-1A,syntaxin, HPC-1; P35-1; STX1; SYN1A; neuron-specific antigen HPC-1; syntaxin 1A,Stx1a,Sap,Neuron-specific antigen HPC-1;Synaptotagmin-associated 35 kDa protein,P35A
Search name
Bovine STX1A ELISA KIT ,Bovine Syntaxin-1A ELISA KIT ,Bovine syntaxin ELISA KIT ,Bovine HPC-1 ELISA KIT ,Bovine P35-1 ELISA KIT ,Bovine STX1 ELISA KIT ,Bovine SYN1A ELISA KIT ,Bovine neuron-specific antigen HPC-1 ELISA KIT ,Bovine syntaxin 1A ELISA KIT ,Bovine Sap ELISA KIT ,Bovine Neuron-specific antigen HPC-1;Synaptotagmin-associated 35 kDa protein ELISA KIT ,Bovine P35A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Syntaxin- 1A, STX1A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to STX1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for STX1A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain STX1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of STX1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验and some B cell malignancies Negative staining: normal B cells CD1d Positive staining (normal): thymus (low levels), bowel ELISA KIT & ANTIBODY OF USCNLIFE
Procedures for In Vitro Production of Bovine Embryos
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in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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