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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Osteopontin,SPP1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Osteopontin, OPN ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Osteopontin,Uropontin,Urinary stone protein,Secreted phosphoprotein 1(SPP-1),Bone sialoprotein 1,Nephropontin, osteopontin,secreted
phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); osteopontin/immunoglobulin alpha 1 heavy chain
constant region fusion protein; BNSP; BSPI; ETA-1; OPN; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; urinary
stone protein; uropontin; secreted phosphoprotein 1
SearCh name
Bovine Osteopontin ELISA Kit, Bovine OPN ELISA Kit, Bovine Uropontin ELISA Kit, Bovine Urinary stone protein ELISA Kit,
Bovine SeCreted phosphoprotein 1 ELISA Kit, Bovine SPP-1 ELISA Kit, Bovine Bone sialoprotein 1 ELISA Kit, Dog
Nephropontin ELISA Kit, Bovine bone sialoprotein I ELISA Kit, Bovine early T-lymphoCyte aCtivation 1 ELISA Kit, Dog
immunoglobulin alpha 1 heavy Chain Constant region fusion protein ELISA Kit, Bovine BNSP ELISA Kit, Bovine BSPI ELISA Kit,
Bovine ETA-1 ELISA Kit, Bovine SPP1 ELISA Kit, Bovine CALPHA1 fusion ELISA Kit, Bovine early T-lymphoCyte aCtivation 1
ELISA Kit, Bovine nephropontin; urinary stone protein ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Osteopontin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Osteopontin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Osteopontin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Osteopontin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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