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文献和实验Automated Protein NMR Structure Determination in Solution
The main drawback of protein NMR spectroscopy today is still the extensive amount of time required for solving a single structure. The main bottleneck in this respect is the manual evaluation of the experimental spectra. A clear solution
1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
Microscale thermophoresis (MST) is a new method that enables the quantitative analysis of molecular interactions in solution at the microliter scale. The technique is based on the thermophoresis of molecules, which provides information
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