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文献和实验Protein phosphorylation plays a pivotal role in cell cycle regulation. MPF (M-phase Promoting Factor) and MAPK (Mitogen-activated protein kinase) are two major kinases driving oocyte maturation and early embryonic divisions
Angiotensin Protein Kinase Assay
James Hardwick's angiotensin assay protocol This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use fewer or more cells, depending
Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress
The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that is activated by cellular stresses causing ATP depletion and has been referred to as a “fuel gauge” or “metabolic sensor” of the eukaryotic cell
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